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| Mahe, Kelig; Bellamy, Elise; Lartaud, Franck; De Rafelis, Marc. |
| This work focuses on investigating the potential of calcein and manganese as growth markers of the common cockle (Cerastoderma edule) in the bay of Somme (France). Recapture of shells, previously marked using a chemical marking and then bred in natural conditions, was performed in order to determine the shell growth patterns. Calcein marking has shown a fluorescent increment in shells after only 30 min immersion time at 150 mg L-1, but also for shells immersed 3 h at 50 mg L-1. Likewise, manganese shell marking was revealed under cathodoluminescence for shells immersed 1 h at 120 mg L-1 as well as for shells which spent 4 h at 90 mg L-1. A numerical analysis performed on each marked cockles has revealed 23 micro-increments between the mark and the ventral... |
| Tipo: Text |
Palavras-chave: Calcein; Manganese; Marking; Cathodoluminescence; Shellfish; Cerastoderma edule. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00025/13649/10758.pdf |
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| Langlet, D; Alunno-bruscia, Marianne; Rafelis, M; Renard, M; Roux, M; Schein, E; Buestel, Dominique. |
| We present a cathodoluminescence (CL) study of growth layer deposition in the shell of the oyster Crassostrea gigas. CL is based on the physical properties of lattice-bound manganese (Mn2+), which is the main activator in calcium carbonate. Our study involved chemical marking by immersing individuals in seawater to which manganese chloride had been added, and subsequent reading of the shell with CL microscopy coupled with numeric treatment of microphotographs; CL emission was analyzed using a scanning electron microscope coupled to a spectrometer. Since the marking did not harm the oysters, repeated markings were possible, allowing validation of the inferences made from analysis of the shell rings. Oysters reared in a French Mediterranean lagoon (Thau... |
| Tipo: Text |
Palavras-chave: Mediterranean lagoon; Manganese marking; Carbonate biomineraliation; Shell growth; Cathodoluminescence; Oyster. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1979.pdf |
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